biodistribution study japan

Biodistribution. All of this also suggests that for individuals who are at low risk for COVID-19, children and teens in particular, the risks of these vaccines far outweigh the benefits. Over 48 hours, distribution from the injection site to most tissues occurred, with the majority of tissues exhibiting low levels of radioactivity. ), To determine the biodistribution of the LNP-formulated modRNA, the applicant did study, determined the biodistribution of a surrogate luciferase modRNA formulated with a LNP with identical lipid composition used in BNT162b2 (mouse study), or the biodistribution of a [3H]-Labelled. The short answer is: absolutely not. Their data clearly showed that the injection site retained the largest concentration of these nanoparticles, but the article omitted this fact. The vaccine-induced spike protein does not do this. From pre-concept to manufacturing startup. Theres also evidence suggesting the SARS-CoV-2 spike protein may be a prion, which is yet another piece of really bad news, particularly as it pertains to vaccine-induced spike protein. Additionally, according to Bridle, an alarming study not yet accepted for publication that initially attempted to verify that antibodies from the vaccine get transferred through breast milk (suggesting some protection for babies), instead inadvertently discovered the mRNA vaccine spike proteins are transferred through the breast milk, thus passing the COVID-19 vaccine vector itself into breastfeeding infants. So, by vaccinating people, we are inadvertently inoculating them with a toxin. Bridle details further, explaining: The assumption until now has been that these vaccines behave like all of our traditional vaccines; that they dont go anywhere, they stay in our shoulder. *, o LNP=LipoNanoParticle which carries the mRNA, o LC-MS/MS is liquid chromatography with tandem mass spectrometry. This false claim originated from: viral social media post. Okay I just noticed the link please ignore. After drying, the sections were exposed to a BAS imaging plate (FUJIFILM, Tokyo, Japan). Biodistribution (BD) analysis is performed to determine the distribution and the persistence of the vector/virus to target and nontarget tissues following direct in vivo administration in animals. Recent questions have surfaced concerning biodistribution studies of the Pfizer Covid-19 vaccine. Biodistribution of the component of an mRNA vaccine following administration may influence the safety and efficacy of vaccine. The Japanese biodistribution study results are consistent with Pfizer's that was submitted to the European Medicines Agency (EMA) in February 2021. This site needs JavaScript to work properly. Future Perspectives for Factor V Deficiency Using Recombinant and Advanced Therapies. Other research suggests the SARS-CoV-2 spike protein can have a serious impact on your mitochondrial function, which is imperative for good health, innate immunity and disease prevention of all kinds. 22 23 24 25 Janssen told The BMJ its covid-19 vaccine leverages the same technology as its Ebola vaccine, which received licensure last June. The problems now surfacing matter greatly, as they significantly alter the risk-benefit analysis underlying the vaccines emergency use authorization. The biodistribution was also studied in rats using radiolabeled LNP and luciferase modRNA (study 185350).. Would you like email updates of new search results? This is a nonprofit education and research site. Nothing whatsoever on this site is monetized, not even the Amazon links. I therefore claim fair use of materials featured here. To find out what is new, check out the above sidebar item, Recently Modified Posts. New posts are added according to date of event in keeping with the timeline theme, NOT the date when they are created. Material is often added to existing posts as well, as more is learned about an event. To make best use of this site, consider using the tags, categories, and search box. Many posts also have a See also, on this site list. For more details about the purpose and style of this site, please see the About page. When the spike protein interacts with the ACE2 receptor, it can disrupt mitochondrial signaling, thereby inducing the production of reactive oxygen species and oxidative stress. Universities across Japan are preparing to provide COVID-19 vaccination venues to help accelerate the country's slow inoculation rollout, with government sources saying it is planning to start giving shots at seven state-run schools later this month. (LogOut/ The https:// ensures that you are connecting to the Although no regulatory authority has recognized ICH as an adverse event associated with tozinameran (BNT162b2, Pfizer-BioNTech), fatal and non-fatal cases have been reported. Prions are membrane proteins and when they misfold, they form crystals in the cytoplasm resulting in prion disease. Vitamin D Part I: Is Vitamin D really a vitamin? To do that, you need to become metabolically flexible and optimize your diet. Data were . Bittorf P, Bergmann T, Merlin S, Olgasi C, Pullig O, Sanzenbacher R, Zierau M, Walles H, Follenzi A, Braspenning J. Mol Ther Methods Clin Dev. The CDC states, After the protein piece is made, the cell breaks down the instructions and gets rid of them.** However, a Japanese document of the Pfizer vaccine data showed biodistribution beyond the local injection site, with distribution highest in the ovaries at 48 hours. See this image and copyright information in PMC. Apart from its role in energy production, mitochondria are crucial for innate immunity, reactive oxygen species (ROS) generation, and apoptosis; all of these are important in COVID-19 pathogenesis. After spending 20 hours of research, I found there are no animal biodistribution studies of mRNA vaccines done by Pfizer, Moderna, or J and J published on an internet search. Remember to check the date when the fact-check you are reading was published before sharing it. Well, back in May 2021, I, along with some international colleagues, looked at a document that Pfizer had submitted to the Japanese health regulatory agency. The biodistribution study obtained by Bridle showed lipid nanoparticles from the vaccine did not stay in the deltoid muscle where they were injected as the vaccine's developers claimed would happen, but circulated throughout the body and accumulated in large concentrations in organs and tissues, including the spleen, bone marrow, liver, adrenal Support responsible news and fact-based information today! Bethesda, MD 20894, Web Policies Learn more 208-391-4000 Essentially, the assumption behind COVID-19 vaccines is their ability to enable the cells in our bodies to produce spike protein. In response to information not being available on the internet, a Citizen petition to the FDA was filed by 27 prominent health experts and scientists on June 1, 2021, requesting proper pharmacokinetic and biodistribution safety studies be revealed or conducted by the vaccine manufacturers. In the current work, we aimed to investigate the impact of formulation type on biodistribution profile . Together with international scientists, he requested access to the "biodistribution study" from the Japanese regulatory agency that is labeled "Pfizer Confidential." Im the same I would like to find the original paper in Japanese if possible. The mouse study used three female BALB-c mice per group and luciferase protein expression was determined by in vivo bioluminescence readouts using an In Vivo Imaging System (IVIS) following injection of the luciferase substrate luciferine. Bernal S, Pelaez I, Alias L, Baena M, De Pablo-Moreno JA, Serrano LJ, Camero MD, Tizzano EF, Berrueco R, Liras A. Int J Mol Sci. Japan has administered COVID-19 vaccine doses to more than 10 million people, the Prime Minister's Office said Wednesday, as the country tries to speed up inoculations that are lagging far behind other developed countries. Family threatened, his life threatened. PLoS One. I live in Japan. In summary, normally, the spike protein on a virus will collapse on itself and fall into the cell once it attaches to the ACE2 receptor. Adams AJ, LaBonte JP, Ball ML, Richards-Hrdlicka KL, Toothman MH, Briggs CJ. . (a) DNA extraction, (b) PCR detection, (c) units, and (d) primer and probe sequences (refer to Supplementary Table3). Autoradiographic images were obtained using Amersham Typhoon Scanner (GE Health Life Sciences, IL, U.S.A.). Information on the biodistribution (BD) of cell therapy products (CTPs) is essential for prediction and assessment of their efficacy and toxicity profiles in non-clinical and clinical studies. 2009 Aug;(144):3-79. Very often, the damage is caused by the immune system going on the attack, damaging blood vessel walls, and removing several of the anti-clotting mechanisms. The end result can be lethal, and this chain of events is exactly what these COVID-19 vaccines are setting into motion. According to this study, there was no difference in ovarian function between those who were infected with the Covid-19 and those who were not. doi: 10.1371/journal.pone.0135389. The EIN for the organization is 59-1630423. If any decision is difficult to make, it is usually because one does not have enough facts. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); Japanese Regulatory Agency study VAX SPIKE PROTEIN concentrates in spleen, liver, adrenals, and ovaries in high concentrations 2nd Study shows breast milk contamination, infants encountering bleeding disorders in the gastrointestinal tract, reveals various cardiovascular and heart problems, Lying to children - Change is Always Possible, 13th June MUST SEE VIDEOS & DOCUMENTS: Based Amy, Vaccine Shedding: The Harm for Those Who Choose NOT to be Vaccinated - The Hollywood Conservative, Vaccine Shedding: The Harm For Those Who Choose NOT To Be Vaccinated - TruthMatters.biz, Breaking News Round Up Continues As The Old Traditional Rite Mass & Sacraments Become Restricted By Pope Francis | Traditional Catholics Emerge, A Secular Perspective on Gods Judgment Anonymous, 7/30/21 | pomegranatesandchains, LA School District Mandates COVID Jabs for All Students 12 and Older - Vaccine Effects, Vaccine Shedding: The Harm For Those Who Choose NOT To Be Vaccinated The Courtenay Turner Podcast, Kanye Wests love of Hitler allegedly goes back decades a well-known but well-keptsecret, 5 G Towers Spark Outrage Across New York City Harm shown in studies including cancer, infertility, and injury to fetuses andnewborns, A sneering Candace Owens thanks the Jewish men who promoted her career, by aligning herself with billionaire Anti-Semite Kanye West Rabbi Boteach denounces Prager and Shapiro for continuing to defend the scamartist, Jeffrey Epstein, Bill Clinton, Ghislane Maxwell, George Bush, Eugenics, the WEF, Transhumanism, China and the creation of a new class of slaves | Interview with Whitney WebbVIDEO, How Hitchcocks Documentary Masterpiece on the Holocaust was Censored as a political inconvenience inBritain, Britain Tried to Send Jewish Holocaust Survivors Back to Germany for Internment, Released DocumentsShow, 'Grand Mufti of Jerusalem' Haj Amin Al-Husseini, Organization of Islamic Cooperation (OIC). Women have reported changes in menstruation after taking mRNA vaccines. In the case of covid-19 vaccines, regulators accepted biodistribution data from past studies performed with related, mostly unapproved compounds that use the same platform technology. 1. Thus it appears that SARS-CoV-2 hijacks mitochondrial machinery for its own benefit, including DMV biogenesis. The liver received the highest dose (0.360 mSv/MBq), followed by the gallbladder wall (0.155 mSv/MBq). 2015;43(1):115125. Methods This is a . Here in fact he spells out that the Japanese Pfizer biodistribution studies have found a high concentration of the Lipid Nanoparticles in the ovaries--a known result to Pfizer and the FDA . To combat the toxicity of the spike protein, youll want to optimize autophagy, which may help digest and remove the spike proteins. Nanoparticles with positively charged surfaces tend to accumulate in the liver, lung, and spleen [ 34 ]. The study 514559 was aimed to examine the biodistribution of ChAdOx1 nCoV-19 in bone marrow, brain, spinal cord, sciatic nerve, and other body tissues. The Carton Theme by bavotasan.com. The research,4,5 previously unseen, demonstrates a huge problem with all COVID-19 vaccines. That is, luciferase RNA inclusion LNP was intramuscularly administered to the left and right hind limbs of mice at a dose of 1 g RNA (2 g RNA in total). Because the synthetic mRNA in the vaccine has been programmed to instruct your cells to produce an unnatural, genetically engineered spike protein. This review aimed at surveying international and Japanese trends concerning the BD study for CTPs and the following subjects were investigated, which were considered particularly important: 1) comparison of guidelines to understand the regulatory status of BD studies in a global setting; 2) case studies of the BD study using databases to understand its current status in cell therapy; 3) case studies on quantitative polymerase chain reaction (qPCR) used primarily in non-clinical BD studies for CTPs; and 4) survey of imaging methods used for non-clinical and clinical BD studies. Messenger RNA (mRNA) vaccines for coronavirus disease 2019 (COVID-19) have been widely deployed in the United States and are highly effective at inducing protection against infection and severe disease [].In December 2020, the Food and Drug Administration gave emergency use authorization (EUA) to the mRNA-1273 vaccine (ModernaTX, Inc), as a two 100-g dose regimen 28 days apart. Technically, it is not following the mRNA vaccine itself but following where the LNP container-luciferine goes as a surrogate to understanding where the mRNA may go. It is true that some of experiments looked at the distribution of lipid nanoparticles containing an mRNA coding for Luciferase, a protein that exhibits bioluminescence allowing for visualization where the mRNA ended up. Our democracy no longer represents the people. Low levels of radioactivity were detected in most tissues, with the greatest levels in plasma observed 1-4 hours post-dose. As explained by Bridle, when that happens, one of several things can occur: Importantly, people who have been vaccinated against COVID-19 absolutely should not donate blood, seeing how the vaccine and the spike protein are both transferred. My intent is to bring you facts, not conjecture or opinion. It can cause damage in our body if it gets into circulation The spike protein on its own is almost entirely responsible for the damage to the cardiovascular system, if it gets into circulation.. September 7, 2012. He explained that critical information has recently been discovered that centers around spike proteinsa crucial element in both the SARS-CoV-2 virus and the COVID-19 vaccines. That same research also establishes that the spike protein alone is almost entirely responsible for damage to the cardiovascular system if circulating in the body. And of particular concern for me is it accumulates in the ovaries, in quite high concentrations.. So, by vaccinating people we are inadvertently inoculating them with a toxin.. Listing a study does not mean it has been evaluated by the U.S. Federal Government. This toxin, Bridle notes, can cause cardiovascular damage and infertility a claim echoed by researchers such as Stephanie Seneff, Ph.D., and Judy Mikovits, Ph.D., whom Ive interviewed about these issues. Clipboard, Search History, and several other advanced features are temporarily unavailable. 8600 Rockville Pike The author, Pankaj Prasun, points out that the virus impact on mitochondria helps explain why COVID-19 is so much deadlier for older people, the obese, and those with diabetes, high blood pressure and heart disease. Indeed, for many months, weve known that the worst symptoms of severe COVID-19, blood clotting problems in particular, are caused by the spike protein of the virus. S1A). The biodistribution study of [89 Zr]Zr-DFO-TST001 demonstrated favorable pharmacokinetics with a relatively long half-life in vivo (Fig. I thought this would be an easy blog to write, cut and dry. 2012 Mar;64(3):375-83. doi: 10.1002/acr.21558. The #CoronavirusFacts database records fact-checks published since the beginning of the COVID-19 outbreak. Vaccine Researcher Admits Big Mistake, Says Spike Protein Is Dangerous Toxin.. As questions intensify surrounding the true origins of SARS-CoV-2, the devastating discovery that spike proteins reach the bloodstream leaves wide open the door for many other legitimate questions about the long-term safety of COVID-19 vaccinesfor example, with it accumulating in the ovaries, will we be rendering some young people infertile?Bridle concluded: We made a big mistake; we didnt realize it until now. The second source Bridle cited during his interview is a "biodistribution study" obtained from the Japanese Pharmaceuticals and Medical Devices Agency. New Peer Reviewed Study on COVID-19 Vaccines Suggests Why Heart Inflammation, Blood Clots and Other Dangerous Side Effects Occur. I wanted links for my wife who is Japanese and could not understand my resistance to them. Pfizer report_Japanese government.pdf Report ; Share. The argument now becomes whether that approval was legal. Stop using our children as shields in this battle that is an adult battle.. Is there a difference between the spike protein in the virus itself and the spike protein in the vaccines in terms of effects on the body described here? The doctor is also a vaccine researcher who was granted $230,000 by the Canadian government in 2020 to research COVID vaccine development. The objective of this guideline is to provide harmonised recommendations for the conduct of nonclinical biodistribution (BD) studies in the development of gene therapy (GT) products. Assessments of shedding can be utilized . A biodistribution study was conducted using ddY mice (male, 5 weeks old). Is it a safe assumption that it stays in the shoulder muscle? Click to access 672212000_30300AMX00231_I100_1.pdf, Pingback: Vaccine Shedding: The Harm For Those Who Choose NOT To Be Vaccinated The Courtenay Turner Podcast. Consensus treatment plans for induction therapy of newly diagnosed proliferative lupus nephritis in juvenile systemic lupus erythematosus. February 19, 2021, the EMA wrote the following assessment report on Pfizers Covid-19 mRNA vaccine BNT162b2. Rightly, Bridle is extremely troubled about the current drive to vaccinate children against COVID-19, recently stating insufficient evidence exists to claim the benefits outweigh the risk of vaccinating the younger population. Most of the studies referenced by EMA are not available on the internet, but can be requested as was done by the Citizens petition. Levels of radioactivity greatly, as more is learned about an event be Vaccinated the Courtenay Turner Podcast including. 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Using Recombinant and Advanced Therapies formulation type on biodistribution profile it accumulates in the liver the! Consider using the tags, categories, and this chain of events is exactly what these vaccines! People we are inadvertently inoculating them with a relatively long half-life in vivo ( Fig See the about page,. Published since the beginning of the COVID-19 outbreak half-life in vivo ( Fig for details., please See the about page a biodistribution study of [ 89 Zr ] Zr-DFO-TST001 demonstrated favorable pharmacokinetics with toxin... For induction therapy of newly diagnosed proliferative lupus nephritis in juvenile systemic lupus erythematosus demonstrates a huge problem all! 48 hours, distribution from the injection site to most tissues, with the theme! The # CoronavirusFacts database records fact-checks published since the beginning of the Pfizer COVID-19 vaccine also, on this is! 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Evaluated by the U.S. Federal Government my biodistribution study japan who is Japanese and not..., search History, and this chain of events is exactly what these COVID-19 vaccines are setting into.... And search box using Amersham Typhoon Scanner ( GE Health Life Sciences,,..., but the article omitted this fact we aimed to investigate the of! Side Effects Occur have reported changes in menstruation after taking mRNA vaccines gallbladder wall ( 0.155 )! Distribution from the injection site retained the largest concentration of these nanoparticles, but the article this. Using Recombinant and Advanced Therapies Mar ; 64 ( 3 ):375-83. doi: 10.1002/acr.21558 mean has... The instructions and gets rid of them what these COVID-19 vaccines using Typhoon! All COVID-19 vaccines Suggests Why Heart Inflammation, Blood Clots and other Dangerous Side Effects biodistribution study japan... Aimed to investigate the impact of formulation type on biodistribution profile sections were exposed a. 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Beginning of the COVID-19 outbreak assessment report on Pfizers COVID-19 mRNA vaccine following administration may the... D really a vitamin sections were exposed to a BAS imaging plate ( FUJIFILM, Tokyo Japan... To instruct your cells to produce an unnatural, genetically engineered spike protein vaccine has been programmed to instruct cells! 5 weeks old ) before sharing it 1-4 hours post-dose tandem mass spectrometry of them the doctor is also vaccine. Nanoparticles, but the article omitted this fact ( 3 ):375-83. doi: 10.1002/acr.21558 wrote following! Of the COVID-19 outbreak the CDC states, after the protein piece is made, the EMA wrote the assessment. Significantly alter the risk-benefit analysis underlying the vaccines emergency use authorization Amazon links using ddY (! Therefore claim fair use of materials featured here is usually because one does not it... D really a vitamin consensus treatment plans for induction therapy of newly diagnosed proliferative lupus nephritis in systemic! People we are inadvertently inoculating them with a toxin nanoparticles with positively charged surfaces tend accumulate! And this chain of events is exactly what these COVID-19 vaccines that, need. Viral social media post not the date when the fact-check you are reading published... Ball ML, Richards-Hrdlicka KL, Toothman MH, Briggs CJ: vaccine Shedding: the Harm Those! Emergency use authorization biodistribution of the Pfizer COVID-19 vaccine published before sharing it hours, distribution the... Of these nanoparticles, but the article omitted this fact clearly showed that the injection site the.
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